For men on anabolic steroids, regular blood testing is essential to monitor potential side effects and maintain long-term health. Liver function markers (ALT, AST, ALP, GGT, albumin, total protein, and bilirubin) help assess how well the liver is handling the increased metabolic stress from steroid use. Since steroids can impact iron metabolism, testing iron, ferritin, transferrin, and transferrin saturation ensures proper oxygen transport and prevents iron overload, which can contribute to oxidative stress. Inflammation markers like CRP, homocysteine, IL-6, and ferritin help identify increased cardiovascular risk, as steroid use can elevate systemic inflammation and strain the heart.

Electrolytes and kidney function markers, including sodium (Na), potassium (K), chloride (Cl), bicarbonate (HCO3), urea, eGFR, and creatinine, are crucial for assessing hydration status, kidney function, and overall metabolic balance, as steroid use can affect fluid retention and kidney stress. Lipid panel markers—cholesterol, triglycerides, HDL, LDL, and risk ratios—help determine cardiovascular risk, since steroids

often lower HDL (the "good" cholesterol) and raise LDL and triglycerides, increasing the risk of heart disease. Insulin and blood sugar balance should also be monitored, as anabolic steroids can contribute to insulin resistance, increasing the risk of metabolic dysfunction.

Hormone testing is crucial to assess endocrine function. Testosterone and DHEA levels provide insight into overall androgen status, while estradiol (E2) monitoring helps detect excessive estrogen conversion, which can lead to side effects like water retention and gynecomastia. Prolactin levels should be checked, especially when using compounds like nandrolone (Deca) or trenbolone, as elevated levels can cause unwanted estrogenic effects. LH and FSH are important markers of natural testosterone production, which is often suppressed on cycle and can help assess recovery post-cycle. Regular testing of these markers allows for early detection of potential issues, helping to manage side effects and optimize overall health, whether on cycle or during post-cycle recovery.